This is a knockout-validated antibody summary, based on the publication "Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (a) Western blot on cell lysates originating from single cell clones after CRISPR-Cas9 treatment. PAX6 Control cells have undergone CRISPR-Cas9 treatment and single cell sorting but still express PAX6. Detection by anti-PAX6 antibody. Actin staining was used for loading control. KO, knock out; WT, wild type U251 N; L, Molecular weight marker. (b) Immunocytochemistry (ICC) on WT U251 N and pooled PAX6 KO cell lines (2A.3, 2A.28 and 2.10). Anti-PAX6 antibody detect endogenous PAX6 protein, DAPI staining identifies the nucleus. Scale bars indicate 10 μm. From [1].

Mouse monoclonal IgG1, kappa light

Company: Developmental Studies Hybridoma Bank

Antibody: PAX6

Catalog number: PAX6

Summary: Mouse monoclonal IgG1, kappa light chain against recombinant chicken Pax6 partial protein (N-terminal region, aa 1-223). Reacts with amphibian, avian, fish, human, lizard, mouse, opossum, planaria, rat, turtle, and zebrafish. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), function blocking, flow cytometry, and chromatin IP.

Western blot | Immunocytochemistry

WT and Pax6-KO U251 N cells.

IC: 5% BSA, 20 min, RT.

WT: 1:37 dilution.

IC: 1:37 in PBS, 1% BSA, 1 h, RT.

WT: 1:10,000 dilution anti-mouse 800 CW (LI-COR Biosciences, cat#926–32,212) IRDye secondary antibodies in TBST.

IC: 1:1,000 dilution Alexa Fluor® 555- conjugated goat anti-mouse IgG, (Life Technologies, Cat# A-32727).

WT: Images were acquired on Odyssey Sa (LI-COR Biosciences).

IC: Zeiss LSM 780 inverted confocal microscope with a 63 × 1.4 plan-Apochromat NA objective.

The same clone (PAX6) is sold as Santa Cruz Biotechnology sc-81649; Developmental Studies Hybridoma Bank PAX6.