This is a knockout-validated antibody summary, based on the publication "PARP Theranostic Auger Emitters Are Cytotoxic in BRCA Mutant Ovarian Cancer and Viable Tumors from Ovarian Cancer Patients Enable Ex-Vivo Screening of Tumor Response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescent analysis of PARP-1 specific DNA damage induced by [2] KX1 in OVCAR8 wt and PARP1 KO cells. Images of representative single cells treated with [2] KX1 with and without veliparib blocking. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody:

Catalog number: 9532

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry.

Western blot | Immunocytochemistry

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Figure 2. Western blot analysis of DNA damage and PARP-1 activity in isogenic OVCAR8 wildtype (wt) and PARP1 knockout (KO) cells treated with [2] KX1. From [1].

WT and PARP1 KO OVCAR8 cells.

WB: LiCor Odyssey blocking buffer for 1 h.

ICC: 1:1,000 dilution for 1 h.

WB: 1:1,000 dilution in Odyssey blocking buffer for 1h incubation at 37C.

ICC: goat anti-rabbit IgG Alexa Fluor Plus 488 (cat# A327731) for 1 h.

WB: 1:10,000 dilution secondary antibodies (Thermo Fisher, Philadelphia, PA, USA) in Odyssey blocking buffer and incubated at 37C for 1 h.

ICC: Images were taken on a Zeiss Observer Microscope and fluorescent intensity was quantified using Zeiss Zen software (Zeiss, Netherlands).

WB: LiCor imager.

The same clone (46D11) is sold as LifeSpan Biosciences LS-B5991; Cell Signaling Technology 9532, 6704.