PARP-2 antibody | knockout validation | Millipore MABE18

This is a knockout-validated antibody summary, based on the publication "PARP2 mediates branched poly ADP-ribosylation in response to DNA damage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PARP-2 antibody | knockout validation | Millipore MABE18 figure 1
Figure 1. Western blot of PARP2 in WT, PARP2-KO and PARP2-reconstituted U2OS cells, with either full length (FL) or the E545A mutant. Actin was used as the control of cell lysates. From [1].
Antibody information

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: PARP-2

Catalog number: MABE18

Summary: Mouse monoclonal IgG1κ against a recombinant protein corresponding to mouse PARP-2. Reacts with mouse and human. Suitable for western blot, immunoprecipitation, immunohistochemistry and ELISA.

Validation Method

Western blot

Sample

Wildtype, PARP2-KO and PARP2-reconstituted U2OS cells.

Clone note

The same clone (4G8) is sold as Enzo Life Sciences ALX-804-639-L001; EMD Millipore MABE18.

References
  1. Chen Q, Kassab M, Dantzer F, Yu X. PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Nat Commun. 2018;9:3233 pubmed publisher