PADI4 / PAD4 antibody | knockout validation | Abcam ab214810

This is a knockout-validated antibody summary, based on the publication "Neutrophil Activation and NETosis Are the Major Drivers of Thrombosis in Heparin-Induced Thrombocytopenia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

PADI4 / PAD4 antibody | knockout validation | Abcam ab214810 figure 1
Figure 1. Western blot of PAD4 in bone marrow cells from PAD4+/+ and PAD4–/– mice probed with anti-PAD4 antibody. ꞵ-actin was used as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: PADI4 / PAD4

Catalog number: ab214810

Summary: Rabbit monoclonal IgG against a synthetic peptide within Mouse PADI4/ PAD4 aa 50 to the C-terminus. Reacts with mouse, and it is predicted to work with rat. Suitable for western blot and immunohistochemistry. Unsuitable for immunoprecipitation.

Validation Method

Western blot

Sample

Bone marrow cells from PAD4+/+ and PAD4–/– mice.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:2,000 dilution anti-rabbit HRP (P0260; DAKO, Denmark).

Detection

Signals were detected using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific) and an ImageQuant LAS 4000 imager (GE Healthcare).

References
  1. Perdomo J, Leung H, Ahmadi Z, Yan F, Chong J, Passam F, et al. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia. Nat Commun. 2019;10:1322 pubmed publisher