P2X7 Receptor antibody | knockout validation

P2X7 Receptor antibody | knockout validation | Alomone APR-004

This is a knockout-validated antibody summary, based on the publication "Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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P2X7 Receptor antibody | knockout validation | Alomone APR-004 figure 1

Figure 1. Western blot analysis of P2X7R in cell free supernatants from wild-type (P2rx7+/+) or P2X7R-deficient (P2rx7-/-) mouse bone marrow-derived macrophages (BMDMs). CathpB levels serve as loading control. From [1].

Antibody information

Rabbit polyclonal IgG

Company: Alomone

Antibody: P2X7 Receptor

Catalog number: APR-004

Summary: Rabbit polyclonal IgG against an epitope of rat P2X7 Receptor. Reacts with rat, mouse, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry.

Validation Method

Western blot

Sample

Cell free supernatants from wild-type (P2rx7+/+) and P2X7R-deficient (P2rx7-/-) mouse bone marrow-derived macrophages (BMDMs).

Primary incubation

1:200 to 1:2,000 dilution in blocking buffer overnight at 4 C.

Secondary incubation

1:5,000 dilution HRP-conjugated secondary anti-rabbit antibodies (GE Healthcare).

Detection

Pierce ECL2 kit and Thyphoon 9410 scanner.

References

de Torre-Minguela C, Barberà-Cremades M, Gómez A, Martín-Sánchez F, Pelegrin P. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep. 2016;6:22586 pubmed publisher

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