This is a knockout-validated antibody summary, based on the publication "Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blots of mouse retina (D) and cortex (E). An N-terminal specific P2X7-R antibody detected the protein transcript of variant 1 mRNA in WT samples 1 and 2 but not P2X7-KO samples 3 and 4 (68.41 kDa) of both retina and cortex. Protein for variant 2/3 (50.69 kDa/49.38 kDa) and 4 (17.2 kDa) was expressed in both WT and P2X7-KO retina and cortex. GAPDH was used as loading control. From [1].

Rabbit polyclonal

Company: Alomone labs

Antibody: P2X7-R

Catalog number: APR008

Summary: Rabbit polyclonal against against an epitope of the mouse protein. It recognizes the extracellular N-terminal region P2X7 from mouse, rat, and human samples. Suitable for western blot, indirect flow cytometry, immunohistochemistry, and live cell imaging applications.

Western blot

Retinae and cortex from both P2X7-KO and WT mice.. Tissues were dissected and homogenised in a HEPEs buffer containing 40 mM HEPEs, 320 mM sucrose, pH 7.5, and a protease inhibitor cocktail.

0.5% skim milk powder in Tris-buffered saline with Tween 0.05% (TBST).

1∶150 dilution for 10 min in a SNAP i.d. system (Millipore).

1∶3,000 diluted goat anti-rabbit IgG-680CW (Odyssey-Licor-Millenium; Cat no#926-68021) for 10 min.

OdysseyClx Infrared imaging system (Licor biosystems).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.