Orai1 antibody | knockout validation | Abcam ab175040

This is a knockout-validated antibody summary, based on the publication "Orai1 Channels Are Essential for Amplification of Glutamate-Evoked Ca 2+ Signals in Dendritic Spines to Regulate Working and Associative Memory", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Orai1 antibody | knockout validation | Abcam ab175040 figure 1
Figure 1. Immunohistochemistry reveals significant expression of the Orai1 protein in the CA1, CA3, and dentate gyrus. SO: Stratum Oriens, SR: Stratum Radiatum, SP: Stratum Pyramidale, SGZ: subgranular zone, GL: granule cell layer; ML: molecular layer. Orai1 staining is lost in littermate KO mice (Orai1fl/fl Nes-Cre). Image shows the CA1 region. Scale bar, 50 µm. From [1].
Antibody information

Mouse monoclonal IgG

Company: Abcam

Antibody: Orai1

Catalog number: ab175040

Summary: Mouse monoclonal antibody against Orai1. Reacts with rat and human. It is predicted to work with mouse and Cynomolgus monkey. Suitable for western blot and immunohistochemistry (paraffin).

Validation Method

Immunohistochemistry

Sample

Control and Orai1-KO mouse brain tissues.

Blocking agent

10% NGS, 0.25% triton, and 1%BSA.

Primary incubation

Diluted in blocking solution.

Secondary incubation

1:500 dilution anti-mouse Alexa 488 (Invitrogen) in blocking solution with 5% NGS at RT for 90min.

Detection

Confocal imaging was performed on a Nikon A1R upright confocal microscope.

References
  1. Maneshi M, Toth A, Ishii T, Hori K, Tsujikawa S, Shum A, et al. Orai1 Channels Are Essential for Amplification of Glutamate-Evoked Ca2+ Signals in Dendritic Spines to Regulate Working and Associative Memory. Cell Rep. 2020;33:108464 pubmed publisher