Oct-1 antibody | knockout validation

Oct-1 antibody | knockout validation | Cell Signaling 8157S

This is a knockout-validated antibody summary, based on the publication "An Argonaute phosphorylation cycle promotes microRNA-mediated silencing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Oct-1

Catalog number: 8157S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ala367 of human Oct-1 (POU2F1) protein. Reacts with human by western blot, immunoprecipitation (IP), and chromatin IP.

Validation Method

Western blot

Sample

Wildtype and POU2F1 knockout HCT116 cells.

Figure

Western blot analysis confirming loss of expression of POU2F1 in HCT116 knockout clones. Please see Extended Data Figure 2d in the article [1].

References

Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;: pubmed publisher

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