This is a knockout-validated antibody summary, based on the publication "The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Cell Signaling
Antibody: OTULIN
Catalog number: 14127
Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues near the amino terminus of human OTULIN protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation. Predicted to react based on 100% sequence homology with pig.
Western blot
Control and Otulin-KO bone marrow derived macrophages. Cells were lysed directly in sample buffer (50 mM Tris pH 6.8, 10% glycerol (v/v), 100 mM DTT, 2% SDS (w/v), bromophenol blue).
5% milk in PBS-T (PBS + 0.1% (v/v) Tween-20) for 30 min.
Primary antibody diluted in PBS-T + 3% BSA at 4°C overnight.
Anti-rabbit IgG-HRP (GE Healthcare, NA934) at room temperature for 1 hr.
Amersham Western Blotting Detection Reagent (GE Healthcare) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.