This is a knockout-validated antibody summary, based on the publication "Disruption of the Golgi protein Otg1 gene causes defective hormone secretion and aberrant glucose homeostasis in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of OTG1 in E13.5 embryos and tissues of P1 wild type, Otg1+/PB and Otg1PB/PB mice. β-actin was used as loading control. From [1].

Rabbit polyclonal

Company: Sigma

Antibody: OTG1

Catalog number: HPA018019

Summary: Rabbit polyclonal against human coiled-coil domain containing 186 (OTG1). Reacts with human, mouse, and rat. Suitable for immunoblotting, immunofluorescence and immunohistochemistry.

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Figure 2. Immunofluorescence staining of OTG1 (in red) protein and Giantin (in green) in both pancreatic tissues and MEFs from wild-type and Otg1PB/PB mice. Nuclei were stained with DAPI. From [1].

Western blot | Immunohistochemistry | Immunocytochemistry

Tissues and MEFs from wild type and OTG1-KO mice. WB: Lysates were prepared with RIPA lysis buffer. IHC: Pancreas from WT and OTG1-KO mice were fixed in 4 % paraformaldehyde overnight and frozen sections cut. ICC: MEFs cells from WT and OTG1-KO mice.

WB: 1:1,000 dilution.

IHC/ICC: 1:1,000 dilution.

WB: 1:5,000 dilution goat anti-rabbit IgG-HRP (Santa Cruz, sc-2004).

IHC/ICC: 1:2,000 dilution donkey anti-rabbit IgG-Cy3 (Millipore, AP182C).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.