This is a knockout-validated antibody summary, based on the publication "IRE1α is an endogenous substrate of endoplasmic-reticulum-associated degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Recombinant rabbit monoclonal
Company: Abcam
Antibody: OS9
Catalog number: ab109510 (Clone EPR4272(2))
Summary: Recombinant rabbit monoclonal raised against a synthetic peptide corresponding to the C-terminal region of human OS9. Supplier recommended for WB, IP, IHC and ICC. Reacts with human, mouse and rat OS9.
Western blot
Lysates of wild-type and OS9 knockout HEK293T cells.
Anti-OS9 diluted 1:10 000.
Image Lab ChemiDOC XRS+ (BioRad).
See Supplemental Figure 2 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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