This is a knockout-validated antibody summary, based on the publication "Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P 2/PI(3,4)P 2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: ORP1
Catalog number: ab131165
Summary: Rabbit monoclonal IgG against residues corresponding to human ORP1 (aa 900-1,000). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry/immunofluorescence, and flow cytometry. Unsuitable for immunoprecipitation.
Western blot
Control, ORP1L KO, and ORP1L/ORP1S DKO HeLa cells.
At 4 °C overnight.
1:5,000 dilution peroxidase-conjugated AffiniPure donkey anti-rabbit IgG (H+L; Jackson ImmunoResearch Laboratories).
ECL Western blotting detection reagent (GE Healthcare or Merck Millipore) and visualized with Molecular Imager® ChemiDocTM XRS + (Bio-Rad Laboratories).
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