This is a knockout-validated antibody summary, based on the publication "Two subunits of human ORC are dispensable for DNA replication and proliferation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Quantitative western blot for ORC2 with an antibody recognizing the N-terminal half of ORC2 in WT (HCT116 p53-/-; ORC2+/+) and two independent ORC2 knockout clones, B2 and BP8. Indicated amount of lysate loaded in each lane. From [1].

Rat monoclonal IgG2a

Company: Santa Cruz

Antibody: ORC2

Catalog number: sc-32734

Summary: Rat monoclonal IgG2a against OCR2, human origin recognition complex (hsORC). Recommended for detection of human ORC2 by western blot and immunoprecipitation.

Western blot

Control (p53-/-; ORC2+/+) and ORC2 knockout HCT116 cells. Cells were lysed in lysis buffer 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 1 mM DTT, 20 mM NaF, and protease inhibitor cocktail.

The same clone (3G6) is sold as Santa Cruz Biotechnology sc-32734, sc-32734 X; ImmuQuest IQ307; Invitrogen MA5-17023; Cell Signaling Technology 4736; LifeSpan Biosciences LS-C82306; EMD Millipore 05-936.