This is a knockout-validated antibody summary, based on two publications "OPA1 deficiency promotes secretion of FGF21 from muscle that prevents obesity and insulin resistance" (discussed below) [2], "Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production" (Figure 1) [1], "OPA1 deletion in brown adipose tissue improves thermoregulation and systemic metabolism via FGF21" for western blot knockout validation (figure 1a) [3] and "Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca2+ homeostasis with adipose tissue lipolysis" for western blot knockout validation (figure s2b) [4]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblotting of OPA1 on cell lysates of mouse neutrophils freshly purified from Opa1N∆ and control mice. From [1].

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: OPA1

Catalog number: 612606

Summary: Mouse monoclonal IgG1 against human (OPA1 aa. 708-830). Reacts with human, mouse, rat, dog, and chicken. Suitable for western blot and immunofluorescence.

Western blot

Gastrocnemius muscle from 12-week-old WT and mOPA1 KO mice. Frozen tissue was homogenized in 200 ul lysis buffer containing (in mmol/l) 50 HEPES, 150 NaCl, 10% glycerol, 1% Triton X-100, 1.5 MgCl2, 1 EGTA, 10 sodium pyrophosphate, 100 sodium fluoride, and 100 umol/l sodium vanadate. Right before use, HALT protease/ phosphatase inhibitors (Thermo Fisher Scientific) were added to the lysis buffer and samples were processed using the TissueLyser II (Qiagen Inc.).

1:1,000 dilution overnight.

IRDye 800CW anti-mouse (1:10,000, LI-COR, Lincoln, NE, USA, #925- 32212) for 1 h.

LiCor Odyssey imager.

Representative immunoblot of OPA1 in gastrocnemius muscle from 12-week-old WT and mOPA1 KO (KO) mice and densitometric analysis of OPA1 normalized by VDAC (n = 6). Please see Figure 1a in the article [2].