This is a knockout-examined antibody summary, based on the publication "Renal PET-imaging with (11)C-metformin in a transgenic mouse model for chronic kidney disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot for OCT2 protein with a wildtype mouse as positive control (lane 1) and a OCT1/2 KO mouse as negative control (lane 2). The band (arrow) corresponding to the positive control in the Tg and WT mice is assumed to represent OCT2 protein abundance. From [1].

Company: LifeSpan Bioscience

Antibody: OCT2

Catalog number: LS-C80615

Summary: Rabbit polyclonal IgG against human SLC22A2 (OCT2). Reacts with human and mouse by western blot.

Western blot, please notice the nonspecific band.

Renal tissue from WT and OCT1/2 KO mouse.

2 % bovine serum albumin solution (Sigma-Aldrich, MO, US) for 2 hours.

1:500 dilution overnight.

HRP-conjugated secondary antibody (Amersham, NJ, US) for 1 hour.

Chemiluminescence (Pierce Supersignal West Dura, Thermo Scientific, IL, US) and ChemiDocTM 134 MP imaging system (BioRad).