This is a knockout-validated antibody summary, based on the publication "Kidney-differentiated cells derived from Lowe Syndrome patient's iPSCs show ciliogenesis defects and Six2 retention at the Golgi complex.", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Lysates from Normal and Lowe syndrome (LS) patient fibroblasts as well as HK2 WT (+/+) and OCRL1 KO (-/-) were resolved by SDS-PAGE and the presence of Ocrl1 was investigated by western blotting using a specific antibody. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: OCRL

Catalog number: sc-393577

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-240 mapping at the N-terminus of human OCRL. Recommended for detection of OCRL of mouse, rat, human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Normal and Lowe syndrome (LS) patient fibroblasts and WT and OCRL1 KO HK2 cell lines.

PBST-milk (137mM NaCl, 10mM Na2HPO4, 2.7mM KCl, 0.1% Tween-20, pH = 7.4, 5% non-fat dried milk) for 1h at room temperature.

1:150 dilution overnight at 4°C.

Horseradish peroxidase-conjugated antibodies for 1h at room temperature.

Chemiluminescence substrate SuperSignal West Femto (Pierce) and Alpha-Innotech imaging system (San Leandro, CA, USA).