This is a knockout-validated antibody summary, based on the publication "Occludin-Knockout Human Hepatic Huh7.5.1-8-Derived Cells Are Completely Resistant to Hepatitis C Virus Infection", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1, kappa

Company: Thermo Fisher

Antibody: OCLN

Catalog number: 33-1500

Summary: Mouse monoclonal IgG1, kappa against a fusion protein consisting of the C-terminal region (~150aa) of human occludin. Reacts with human, mouse, rat and dog. Suitable for western blot, immunocytochemistry/immunofluorescence and ELISA. Published to react also with pig, C. elegans, sheep, and guinea pig, and to be suitable also for immunoprecipitation and immunohistochemistry.

Western blot

WT and OCLN-KO (OKH-4) Huh7.5.1-8 cells.

1:1,000 dilution.

1:2,000 dilution HRP-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories).

Electrochemiluminescence system (GE Healthcare UK Ltd., Little Chalfont, Buckinghamshire, UK)

Please see Figure 1e in the article [1].

The same clone (OC-3F10) is sold as LifeSpan Biosciences LS-C37844, LS-C37846, LS-B2320, LS-C354895, LS-C354896; Invitrogen 33-1520, 33-1500, 331594, 331588, 33-1511.