This is a knockout-validated antibody summary, based on the publication "Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot confirming loss of STN1 protein expression in two independent Stn1 knockout clones (lower panel). An immunoblot for PARP1 is included as a loading control (upper panel). From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: OBFC1

Catalog number: sc-376450

Summary: Mouse monoclonal antibody against amino acids 8-307 mapping at the N-terminus of mouse OBFC1. Recommended for detection of OBFC1 (STN1) of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and STN1 KO mES cells.

1:1,000 dilution.

1:10,000 dilution CF770 goat anti‐mouse IgG (Biotium).

Odyssey CLx infrared imaging scanning system (LI‐COR Biosciences)