OAS1 antibody | knockout validation | Santa Cruz sc-374656
DOI
//dx.doi.org/10.13070/ko.en.7.2121
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: OAS1

Catalog number: sc-374656

Summary: Mouse monoclonal IgG2a (kappa light chain) specific for an epitope mapping between amino acids 337-364 at the C-terminus of human OAS1 p41. Recommended for detection of OAS1 p41 isoform of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and OAS1-KO A549 cell lines.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution HRP-conjugated anti-mouse antibody (Thermo Fisher).

Figure

Please see Figure 1a in the article [1].

References
  1. Li Y, Banerjee S, Wang Y, Goldstein S, Dong B, Gaughan C, et al. Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proc Natl Acad Sci U S A. 2016;113:2241-6 pubmed publisher