OAS1 antibody | knockout validation | Cell Signaling Technology 14498

DOI

//dx.doi.org/10.13070/ko.en.7.2122

Date

2017-02-15

This is a knockout-validated antibody summary, based on the publication "Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal antibody

Company: Cell Signaling Technology

Antibody: OAS1

Catalog number: sc-374656

Summary: Rabbit monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp90 of human OAS1 protien. Recommended for detection of total OAS1 of human origin by western blot, and immunoprecipitation.

Validation Method

Western blot

Sample

WT and OAS1-KO A549 cell lines.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution HRP-conjugated anti-mouse antibody (Thermo Fisher).

Figure

Please see Figure 1a and Fig 6a in the article [1].

References

Li Y, Banerjee S, Wang Y, Goldstein S, Dong B, Gaughan C, et al. Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proc Natl Acad Sci U S A. 2016;113:2241-6 pubmed publisher