O-linked N-acetylglucosamine antibody | knockout validation | Thermo Scientific MA1-072

This is a knockout-validated antibody summary, based on the publication "Diverse metabolic effects of O-GlcNAcylation in the pancreas but limited effects in insulin-sensitive organs in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1

Company: Thermo Scientific

Antibody: O-linked N-acetylglucosamine

Catalog number: MA1-072

Summary: Mouse monoclonal IgG1 against pore complex-lamina fraction purified from rat liver nuclear envelopes. Suitable for western blot, immunoprecipitation (IP), immunohistochemistry (paraffin), immunofluorescence, ChIP, dot blot and ELISA.

Validation Method

Western blot

Sample

Pancreatic islets from control Ogt-flox and beta cell-specific Ogt-KO mouse.

Primary incubation

1:1,000 dilution.

Secondary incubation

Horseradish peroxidase-linked antibodies.

Detection

Chemiluminescence.

Figure

Representative western blot analysis for O-GlcNAcylation (RL2) levels in isolated islets from control and beta cell-specific Ogt-βKO mouse. Please see Figure 3b in the article [1].

References
  1. Ida S, Morino K, Sekine O, Ohashi N, Kume S, Chano T, et al. Diverse metabolic effects of O-GlcNAcylation in the pancreas but limited effects in insulin-sensitive organs in mice.. Diabetologia. 2017;0: pubmed publisher