This is a knockout-validated antibody summary, based on the publication "Diverse metabolic effects of O-GlcNAcylation in the pancreas but limited effects in insulin-sensitive organs in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1, kappa
Company: Abcam
Antibody: O-Linked N-Acetylglucosamine
Catalog number: ab2739
Summary: Mouse monoclonal IgG1, kappa against pore complex-lamina fraction purified from rat liver nuclear envelopes. Suitable for western blot, immunoprecipitation (IP), immunocytochemistry/immunofluorescence, immunohistochemistry (frozen), ChromatinIP, and dot blot.
Immunohistochemistry
Liver, skeletal muscle and pancreas tissues from control Ogt-flox and Ogt-KO mice.
1:200 dilution.
Immunohistochemical analysis of O-GlcNAcylation using anti-OGlcNAc (RL2) antibodies in multiple tissues (original magnification ×100). iWAT, inguinal white adipose tissue; N/D, not determined due to lean phenotype. Please see Figure 1b in the article [1].
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