Nur77 antibody | knockout validation | Cell Signaling 3960

This is a knockout-validated antibody summary, based on the publication "Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Nur77 antibody | knockout validation | Cell Signaling 3960 figure 1
Figure 1. The indicated LS174T cell lines were inoculated subcutaneously into flanks of nude mice. After 10 days, mice were intraperitoneally injected with oxaliplatin (5mg/kg) daily. After 12 days, mice were sacrificed for analysis of protein expressions in tumors. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Nur77

Catalog number: 3960

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Leu535 of human Nur77. Reacts with human. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry (fixed/permeabilized).

Validation Method

Western blot

Sample

Control and Nur77-KO (NR4A1-/-) LS174T cell line tumors.

Blocking agent

5% milk in TBST [2] at room temperature.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:1,000 dilution EasyBlot anti-Rabbit IgG (HRP) (GeneTex, #221666-01).

Detection

ECL Western Blotting Substrate (Advansta, Cat# K-12045-D50).

References
  1. Niu B, Liu J, Lv B, Lin J, Li X, Wu C, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12:2809 pubmed publisher
  2. 10mM Tris-HCl (pH 8.0), 150mM NaCl, and 0.05% Tween-20.