This is a knockout-validated antibody summary, based on the publication "A KRAS-responsive long non-coding RNA controls microRNA processing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Abcam
Antibody: Nucleophosmin
Catalog number: ab10530
Summary: Mouse monoclonal antibody against full length native rat NPM1 protein (purified). The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23. Reacts with mouse, rat, cow, dog, human, African green monkey, and Chinese hamster. Suitable for western blot and immunocytochemistry/immunofluorescence.
Western blot
Control and NPM1-KO H1299 cells.
1:1,000 dilution.
1:5,000 dilution anti-mouse IgG, HRP-linked Antibody (Cell Signaling Technology, 7076).
Western Bright ECL-Spray substrate and ChemiDoc instrument (Bio-Rad).
The same clone (FC82291) is sold as Novus Biologicals NB600-1030; Santa Cruz Biotechnology sc-56622; Abcam ab10530; MilliporeSigma B0556.
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