This is a knockout-validated antibody summary, based on the publication "Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Nprl2

Catalog number: sc-376986

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 1-300 mapping at the N-terminus of human NPRL2. Recommended for detection of NPRL2 of mouse, rat, human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including canine and porcine.

Western blot

WT and NPRL2-KO MEFs. Cells were homogenized in lysis buffer consisting of 150 mM sodium chloride, 50 mM sodium fluoride, 100 μM sodium orthovanadate (pH 10.0), 50 mM sodium pyrophosphate tetrabasic, 10 mM β-glycerophosphate, 5 mM EDTA, 5 mM EGTA, 10 mM HEPES (pH 7.4), 0.2% sodium deoxycholate and 0.5% Triton X-100 supplemented with complete anti-protease cocktail (Roche).

Please see Figure 2a,b in the article [1].