This is a knockout-validated antibody summary, based on the publication "Neuropilin2 regulates the guidance of post-crossing spinal commissural axons in a subtype-specific manner", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Goat polyclonal
Company: R&D Systems
Antibody: Npn2
Catalog number: AF567
Summary: Goat polyclonal against mouse myeloma cell line NS0-derived recombinant rat neuropilin‑2 Gln23-Asp857 (Val809-Asp825 del). Detects mouse and rat protein in direct ELISAs and western blots. Approximately 25% cross‑reactivity with recombinant human Neuropilin-2 is observed, and less than 5% cross-reactivity with recombinant rat Neuropilin-1 is observed.
Immunohistochemistry
Mouse spinal cord cryosections from an E11.5 Npn2 null embryo and Neurog2-tauGFP reporter mice.
10% donkey serum and 0.1% Triton X-100.
15 μg/ml in blocking solution.
Secondary antibodies were from Jackson ImmunoResearch Laboratories, Inc (West Grove, PA, USA).
Epifluorescence images were captured using a Nikon Eclipse TE300 microscope (Nikon, Tokyo, Japan).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.