Noxa antibody | knockout validation | Sigma-Aldrich OP180

This is a knockout-validated antibody summary, based on the publication "Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Noxa antibody | knockout validation | Sigma-Aldrich OP180 figure 1
Figure 1. Whole-cell lysates from RPMI-8226 and NOXA KO cells treated or untreated with TTFA were evaluated for expression of indicated proteins by immunoblot analysis. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Sigma-Aldrich

Antibody: Noxa

Catalog number: OP180

Summary: Mouse monoclonal IgG1 against a recombinant protein consisting of human Noxa. Reacts with human by western blot.

Validation Method

Western blot

Sample

Control and NOXA-KO RPMI-8226 and KMS18 cells.

Blocking agent

5% milk in tris-buffered saline with tween TBST) for 1 h.

Primary incubation

1:1,000 dilution made in BLOK™ Casein in TBS (G-Biosciences, #786-196) overnight.

Secondary incubation

3:10,000 in blocking solution for 1 h.

Clone note

The same clone (114C307) is sold as MilliporeSigma OP180; Santa Cruz Biotechnology sc-56169; EMD Millipore OP180.

References
  1. Bajpai R, Sharma A, Achreja A, Edgar C, Wei C, Siddiqa A, et al. Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma. Nat Commun. 2020;11:1228 pubmed publisher