This is a knockout-validated antibody summary, based on the publication "Parent stem cells can serve as niches for their daughter cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling Technology

Antibody: Notch2

Catalog number: 4530S,4530P

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding residues surrounding Val2332 of human Notch2. Reacts with human and rat. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Western blot

Mouse tracheas from control and secretory progenitor cell-specific Notch2-KO. Tissues were removed, fixed in 4% paraformaldehyde and embedded in OCT for cryosectioning.

1:2,000 dilution.

1:1,000 dilution anti-rabbit-HRP conjugate (170-6514, Bio-Rad) for 1 hour at room temperature followed by tyramide signal amplification; and 1:1,000 dilution streptavidin-594 (S-11227, Life Technologies) for 30 minutes at room temperature.

Olympus FluoView FV10i confocal microscope (Olympus Corporation).

2: Figure

Immunostaining for lineage label YFP (green) in combination with N2ICD (red) on control mice (Tam-treated SCGB1A1-Notch2+/+) and experimental airways (Tam-treated SCGB1A1-Notch2fl/fl). White arrowheads point to lineage labeled cells that had lost Notch2 and therefore do not show N2ICD expression. Please see Extended Data Figure 7c in the article [1].