Notch1 antibody | knockout validation | Cell Signaling 4380

This is a knockout-validated antibody summary, based on the publication "O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Notch1 antibody | knockout validation | Cell Signaling 4380 figure 1
Figure 1. Total cell lysates from parental HEK293T cells or NOTCH1-null cells were subjected to immunoprecipitation (IP) using NOTCH1 ECD antibody (Santa Cruz, H-131, #sc-9170). The immunoprecipitates were analyzed by immunoblotting with NOTCH1 ICD antibody (Cell Signaling, D6F11, #4380). Aliquots of total cell lysates were immunoblotted with β-tubulin antibody. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Notch1

Catalog number: 4380

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gln2487 of human Notch1. Reacts with human, mouse and rat. Suitable for western blot, immunofluorescence/immunocytochemistry and flow cytometry.

Validation Method

Western blot

Sample

Control and NOTCH1-null HEK293T cells.

Secondary incubation

HRP-conjugated goat anti-rabbit IgG (Invitrogen, 65–6120).

Notes

KO validation has a IP step which, although unlikely, it might interfere.

EOGT is aso KO validated (Sigma, HPA019460, Fig 1c).

References
  1. Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, et al. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.. elife. 2017;6: pubmed publisher