This is a knockout-validated antibody summary, based on the publication "Notch1 Pathway Activity Determines the Regulatory Role of Cancer-Associated Fibroblasts in Melanoma Growth and Invasion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Undetectable full-length of Notch1 protein (271 kD) and slight amount of N1IC (120 kD, which was likely presented in non-fibroblasts) in skin tissue of LOFNotch1 mice was exhibited by Western blotting analysis. β–actin was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Notch1

Catalog number: ab52627

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human Notch1 aa 2,500-2,600. Reacts with human, mouse, and cow. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and flow cytometry. Unsuitable for immunoprecipitation.

Western blot

skin tissue samples were homogenized in a RIPA buffer (50 mM Tris-Cl, 150 mM sodium chloride 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, pH 8.0, 1 mM EDTA and protease inhibitor cocktails (Roche).

The same clone (EP1238Y) is sold as Abcam ab52627; EMD Millipore 04-1046.