This is a knockout-validated antibody summary, based on the publication "A validated antibody panel for the characterization of tau post-translational modifications", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblotting using hTg-Tau and Tau-KO brain lysates confirms the specificity for tau antibody. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: Nitrated Tau (Tyr29)

Catalog number: MAB2244

Summary: Mouse monoclonal IgG1κ against a synthetic linear peptide corresponding to the amino acids surrounding and including nitrated Tyr29 of human Tau. Reacts with human by western blot and immunohistochemistry.

Western blot

Brains from hTg-Tau and Tau-KO mice. Lysates were prepared by dounce homogenizer in 350 μl of lysis buffer containing 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1 mM EGTA, 1% Triton-X100 and protease, phosphatase, demethylase (500 μM IOX1, 2 μM Daminozide, 10 μM Paragyline Hydrochloride), deacetylase (10 μM Trichostatin A, 5 mM Nicotinamide), O-GlcNAcase (1 μM Thiamet-G) inhibitors.

5% BSA in 1X TBST.

1:500 dilution in blocking solution overnight at 4 °C.

1:20,000 dilution IRDye Donkey anti-mouse 800 or IRDye Goat anti-rabbit 680 in blocking solution for 2 h at room temperature.

Li-Cor Odyssey CLx imaging station.

The same clone (Tau-nY29) is sold as BioLegend 829801, 829804; Santa Cruz Biotechnology sc-66177; EMD Millipore MAB2244.