Ninjurin2 antibody | knockout validation | Abcam ab172627

This is a knockout-validated antibody summary, based on the publication "Ninjurin 2 overexpression promotes human colorectal cancer cell growth in vitro and in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Ninjurin2 antibody | knockout validation | Abcam ab172627 figure 1
Figure 1. Immunoblot of the indicated proteins in control HT-29 cells, and cells transfected with the lenti-CRISPR/Cas9-KO constructs, with non-overlapping sgRNAs against NINJ2 (“sgRNA1/2”) or the non-sense control sgRNA (“sg-C”). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Ninjurin2

Catalog number: ab172627

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Ninjurin2, aa 50 to the C-terminus (Cysteine residue). Reacts with mouse, rat and human. Suitable for western blot and immunoprecipitation. Unsuitable for immunocytochemistry or immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and NINJ2-KO HT-29 cells.

References
  1. Li G, Zhou L, Yang H, He X, Duan Y, Wu F. Ninjurin 2 overexpression promotes human colorectal cancer cell growth in vitro and in vivo. Aging (Albany NY). 2019;11:8526-8541 pubmed publisher