This is a knockout-validated antibody summary, based on the publication "Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of WT and CRISPR-Cas9 mediated NPC1 knockout HeLa cells. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Niemann Pick C1

Catalog number: ab134113

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Niemann Pick C1 (aa 1250-1350, C terminal). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and flow cytometry.

Western blot

WT and CRISPR-Cas9 mediated NPC1 knockout HeLa cells.

TBS (50 mM Tris/HCl, pH 7.5, 150 mM NaCl) with 5% (w/v) non-fat dry milk for 1 hr.

Overnight at 4°C.

1:20,000 dilution anti-rabbit IgG HRP-conjugated antibodies (GE Healthcare) for 1h.

ECL reagents (GE Healthcare).