This is a knockout-validated antibody summary, based on the publication "Tumor- and osteoclast-derived NRP2 in prostate cancer bone metastases", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot showing the total NFATc1 protein in NRP2WT and NRP2KO osteoclasts at day 3 of treatment with RANKL and M-CSF From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Neuropilin-2

Catalog number: 3366

Summary: Rabbit monoclonal antibody against recombinant protein specific to the amino terminus of mouse Neuropilin-2 protein. Reacts with mouse and rat. Suitable for western blot, immunoprecipitation, and immunohistochemistry (paraffin and frozen).

Western blot

WT and NRP2-KO mouse bone marrow-derived osteoclasts.

5% non-fat dry milk in 1X TBST (1X Tris Buffered Saline, 0.1% Tween-20) for at least 30min.

diluted in 1X PBS overnight at 4°C with continuous shaking at low speed.

conjugated with HRP for 1h in 1X TBST with continuous shaking at low speed at room temperature..

SuperSignal™ West Femto Maximum Sensitivity Substrate and SuperSignal™ Pico Maximum Sensitivity Substrate captured on an X-ray film.