This is a knockout-validated antibody summary, based on the publication "Controlling of glutamate release by neuregulin3 via inhibiting the assembly of the SNARE complex", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Neuregulin-3

Catalog number: sc-390171

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 276-345 mapping within an N-terminal extracellular domain of human Neuregulin-3. Recommended for detection of Neuregulin-3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine and bovine.

Western blot

Hippocampus and cortex from control and Nrg3-KO mice. Brain tissues were homogenized in RIPA buffer (50 mM Tris·HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA) containing 0.5% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 mM Na3VO4, 1 mM

NaF, 1 mM DTT, and protease inhibitor mixture.

1:1,000 dilution.

Enhanced chemiluminescence (32106; Pierce).

Ablation of Nrg3 in GFAP-Nrg3f/f mice. Homogenates of hippocampus and cortex were subjected to Western blotting. Please see Figure 1b in the article [1].