Nestin antibody | knockout validation | BD Biosciences 611658

This is a knockout-validated antibody summary, based on the publication "Nuclear Nestin deficiency drives tumor senescence via lamin A/C-dependent nuclear deformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Nestin antibody | knockout validation | BD Biosciences 611658 figure 1
Figure 1. Immunoblotting of the indicated proteins in control and Nestin-knockout H1299 cells. From [1].
Antibody information

Mouse monoclonal IgG1, κ

Company: BD Biosciences

Antibody: Nestin

Catalog number: 611658

Summary: Mouse monoclonal IgG1, κ against a recombinant protein corresponding to aa. 402-604 of rat Nestin. Reacts with rat and human by western blot. Not recommended for immunofluorescence.

Validation Method

Western blot

Sample

Control and Nestin-knockout H1299 cells. Cells were lysed on ice in RIPA buffer (Millipore, 20-188) supplemented with protease inhibitor cocktail (Roche, 04693116001) and phosphatase inhibitor cocktail (Roche, 04906837001).

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution anti-mouse IgG HRP-linked (Cell Signaling Technology, 7076).

References
  1. Zhang Y, Wang J, Huang W, Cai J, Ba J, Wang Y, et al. Nuclear Nestin deficiency drives tumor senescence via lamin A/C-dependent nuclear deformation. Nat Commun. 2018;9:3613 pubmed publisher