This is a knockout-validated antibody summary, based on the publication "The ubiquitin ligase Nedd4-1 participates in denervation-induced skeletal muscle atrophy in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting of Nedd4-1 in gastrocnemius muscle lysates from representative Nedd4-1 SMS-KO mice (M4, M5, M6) and littermate MyoCre;Nedd4-1+/+ control mice (M1, M2, M3). GAPDH served as a loading control. From [1].

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: Nedd4-1

Catalog number: 611480

Summary: Mouse monoclonal IgG1 against mouse Nedd4 (aa. 173-262). Recommended for western blot and immunofluorescence.

Western blot

Gastrocnemius muscle lysates from Nedd4-1 skeletal muscle-specific-KO mice, in muscle lysis buffer, 5 mM Tris-HCl pH 8.0, 1 mM EDTA, 1 mM EGTA, 1 mM β-mercaptoethanol, 1% glycerol, PMSF (1 mM), leupeptin, aprotinin (10 ug/ml each).

HRP-conjugated secondary antibodies

Biorad ECL (Biorad Laboratories, Hercules, CA, USA) or Immun Star Western C (Biorad Laboratories)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.