This is a knockout-validated antibody summary, based on the publication "SLC34A2 Regulates the Proliferation, Migration, and Invasion of Human Osteosarcoma Cells Through PTEN/PI3K/AKT Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signalling
Antibody: NaPi2b/SLC34A2
Catalog number: 42299
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the amino terminus of human NaPi2b/SLC34A2 protein. Reacts with human by western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.
Western blot
WT and SLC34A2-KO SW1353 and U2OS cells. Cells were lysed with RIPA buffer containing protease inhibitor (CST, 5870S) and phosphatase inhibitor (SunShineBio, SN338, China) on ice for 2 h.
5% skim milk for 1 h at room temperature.
At the recommended dilution ratio for 2 h.
For 1 h.
Enhanced ECL Detection Kit (KeyGen, Nanjing, China).
The deletion of SLC34A2 by CRISPR-Cas9 gene editing system was validated by western blot with anti-SLC34A2 antibody. Please see Figure 3a in the article [1].
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