This is a knockout-validated antibody summary, based on the publication "β1-C121W Is Down But Not Out: Epilepsy-Associated Scn1b-C121W Results in a Deleterious Gain-of-Function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemistry of beta1 and AnkG channels from cortex. Courtesy of the author.

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Na Channel β1 Subunit

Catalog number: 14684

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human sodium channel β1 subunit protein. Reacts with human, mouse and rat. Suitable for western blot and immunoprecipitation.

Immunohistochemistry

Brains and optic nerves from WT and Scn1b−/− mice. Tissues were postfixed (brains overnight, optic nerves for 15 min) in 4% PFA, and cryoprotected in 10% sucrose followed by 30% sucrose overnight, flash frozen in 2-methylbutane, and stored at −80°C. Optic nerves were embedded in O.C.T. (Tissue-Tek) before freezing.

10% goat or donkey serum as appropriate, 0.3% Triton X-100, 0.1M phosphate buffer for ≥2 h in a humidified chamber.

1:25-1:250 dilution (for optic nerves and brains, respectively) in blocking buffer overnight in a humidified chamber.

1:500 dilution Alexa Fluor 488-conjugated goat or donkey anti-rabbit secondary antibodies (Thermo Fisher Scientific) diluted in blocking buffer for 2 h in the dark.

Nikon A1R confocal microscope with Nikon NIS-Elements Advanced Research software using either a 60× numerical aperture (NA) 1.40 oil objective or a 20× NA 0.75 dry objective.

Immunohistochemistry of sodium channel β1 subunit (green) in optic nerves and brain from WT, Scn1b+/− and Scn1b-/- mice. Please see Figures 5-7 in the article [1].

The article lists the correct clone number (wrong catalog number) to indicate the antibody (from the author's feedback).