NTH1 antibody | knockout validation | R&D Systems MAB2675
This is a knockout-validated antibody summary, based on the publication "Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Western blot analysis of NTHL1 expression in normal and NTHL1KO organoids. GAPDH, loading control. Courtesy from the Authors [1].
Antibody information
Mouse monoclonal IgG1
Company: R&D Systems
Antibody: NTH1
Catalog number: MAB2675
Summary: Mouse monoclonal IgG1 against recombinant human NTH1
(aa Met1-Leu312). Reacts with human by western blot.
Validation Method
Western blot
Sample
Control and NTHL1-knockout human colon organoids.
Primary incubation
1:250 dilution overnight at 4 C.
Secondary incubation
1:5,000 dilution polyclonal rabbit anti-mouse IgG-HRP (DAKO , P0161) for 2h at room temperature.
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