This is a knockout-validated antibody summary, based on the publication "Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: NT4

Catalog number: sc-365444

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 77-103 within an internal region of NT-4 of human origin. Recommended for detection of NT-4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and canine, bovine and porcine.

Immunocytochemistry

WT and NT4−/− mouse primary pulmonary mast cells.

1:200 dilution.

ICC: 1:500 dilution donkey anti-mouse 546 (Life Technologies, A10036).

ICC: Axiovert 100M LSM 510 microscope (Zeiss)

Please see Figure 4c in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.