This is a knockout-validated antibody summary, based on the publication "The Enhanced Inhibitory Effect of Estrogen on PD-L1 Expression Following Nrf2 Deficiency in the AOM/DSS Model of Colitis-Associated Cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Validation of the Nrf2 protein expression in whole protein extracted from colon tissues of WT and Nrf2 KO male and female mice. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: NRF2

Catalog number: 12721

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Ala275 of human NRF2 protein. Reacts with human, mouse, and monkey. Suitable for western blot, immunoprecipitation (IP), immunofluorescence/immunocytochemistry, flow cytometry, and Chromatin IP.

Western blot

WT and Nrf2 KO mouse colon tissues.

5% bovine serum albumin (BSA) in TBST (20 mM Tris, 150 mM NaCl, containing 0.1% Tween-20, pH 7.4) for 1 h.

1:2,000 dilution in TBST with 5% BSA at 4°C overnight.

Horseradish peroxidase (HRP)-conjugated secondary antibody at room temperature for 1 h.

Enhanced chemiluminescence (ECL) reagent and visualized using a ChemiDoc MP (Bio-Rad).