This is a knockout-validated antibody summary, based on the publication "NOVA2-mediated RNA regulation is required for axonal pathfinding during development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of NOVA1 and NOVA2 proteins in extracts of mouse cortex from age-matched P0 wild-type, Nova2-/-, and Nova1-/- mice with anti-pan NOVA (POMA antisera), anti-NOVA1 specific, anti-NOVA2 specific, anti-PTBP2, and anti-GAPDH antibodies. From [1].

Rabbit monoclonal IgG

Company: abcam

Antibody: NOVA1

Catalog number: ab183024

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Nova1 aa 1-100. Reacts with mouse, rat, and human. Suitable for western blot and immunohistochemistry (paraffin).

Western blot | Immunohistochemistry

Figure 2. Immunohistochemistry of coronal brain sections of E16.5 wild-type, Nova1-/-, and Nova2-/-, stained for NOVA1 (green), NOVA2 (red), and DAPI (blue). Scale bar; 500 μm. From [1].

WB: Extracts of mouse cortex from age-matched P0 wild-type, Nova2-/-, and Nova1-/- mice. IHC: E16.5, E18.5 mice brains were fixed with 4% paraformaldehyde (PFA)/PBS at 4 C overnight, and embedded in OCT compound for cryosection (80 μm, CM3050S, LEICA).

IHC: 1.5% normal donkey serum (NDS)/PBS at RT.

IHC: overnight at 4 C.

IHC: 1:1,000 dilution Alexa 488, 555, or 647 conjugated donkey secondary antibodies in 1.5% NDS/PBS.

IHC: Images of immunostained specimens were collected by BZ-X700 (KEYENCE), fluorescence microscopes Axioplan 2 (Zeiss), or an inverted TCS SP8 laser scanning confocal microscope (LEICA).