This is a knockout-validated antibody summary, based on the publication "Inflammation increases NOTCH1 activity via MMP9 and is counteracted by Eicosapentaenoic Acid-free fatty acid in colon cancer cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analyses for Notch1 Full Length in HT29 CTRL and HT29 Notch1-/- cells. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1,κ

Company: Biolegend

Antibody: NOTCH1

Catalog number: 629101

Summary: Mouse monoclonal IgG1,κ against Notch1 GST fusion protein. Reacts with human and mouse protein in western blot and immunohistochemistry.

Western blot

Control and HT29 Notch1-/- cells.

Nonfat dry milk.

1:1,000 dilution overnight at +4 °C.

Chemiluminescent Sensitive Plus HRP (BioFx Laboratories, MD, United States) and images were acquired with the ChemidocTM XRS+ (Bio-Rad Laboratories).

The same clone (mN1A) is sold as Bio-Rad MCA2410GA, MCA2410A647, MCA2410F; BioLegend 629101, 629102, 629103, 629104, 629105, 629106; Novus Biologicals NB100-78486; OriGene TA328031, TA336494; Santa Cruz Biotechnology sc-32745; LifeSpan Biosciences LS-C40801, LS-C106230, LS-C106459, LS-C106611, LS-C188303, LS-C204312, LS-C204395, LS-C204456, LS-C359244; Abnova MAB11063; Invitrogen MA1-81888, MA5-16862, 41-3500; eBioscience 12-5785-82, 14-5785-81; GeneTex GTX43375; Antibodies-online ABIN120355; Sigma-Aldrich N6786; BD Biosciences 552768, 552466; EMD Millipore MAB5352, FCMAB260PE.