This is a knockout-validated antibody summary, based on the publication "Increased VEGF‐A promotes multiple distinct aging diseases of the eye through shared pathomechanisms", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. A) LRP3 is expressed in RPE cells at sites of choroidal neovascularization lesions (arrow; white). CD31(in red) is used as a blood vessel marker, and phalloidin (in green) to label the cytoskeleton. Scale bar, 100 μm. B) Lack of NLRP3 staining in VEGF‐Ahyper/Nlrp3−/− mice (control). Scale bar, 100 μm. From [1].

Rabbit polyclonal IgG

Company: Santa Cruz Biotechnology

Antibody: NLRP3

Catalog number: H66

Summary: Rabbit polyclonal IgG against an epitope corresponding to amino acids 25-90 mapping near the N-terminus of human cryopyrin. Recommended for the detection of cryopyrin in mouse, rat and human. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, canine, bovine and porcine.

Immunofluorescence

VEGF‐Ahyper and VEGF‐Ahyper/Nlrp3−/− mice eyes.

5% serum.

1:50 dilution overnight at 4°C.

Alexa‐555, or Alexa‐647 antibodies (Life Technologies) at 1:100 dilution incubated for 3 h at room temperature in the dark.

Zeiss epifluorescence microscope (Carl Zeiss Microscopy, Jena, Germany) or Nikon confocal microscope (Nikon Instruments Inc, Melville, NY).