NLRP3 antibody | knockout validation | AdipoGen AG-20B-0014 (cryo-2)

This is a knockout-validated antibody summary, based on the publications "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component", as cited below [1], and "Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis" for western blot knockout validation (figure 1d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: AdipoGen

Antibody: NLRP3

Catalog number: AG-20B-0014 (cryo-2)

Summary: Mouse monoclonal antibody raised against a recombinant polypeptide corresponding to residues 1-93 of mouse NLRP3.

Supplier recommended for WB, IP, IHC and ChIP.

Reacts with human and mouse NLRP3.

Validation Method

Western blot

Sample

Lysates of peritoneal macrophages from wild-type and NLRP3 -/- mice.

Primary incubation

Anti-NLRP3 antibody.

Figure

See Figure 4 in [1].

References
  1. Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol. 2016;17:250-8 pubmed publisher
  2. Jin Y, Liu Y, Xu L, Xu J, Xiong Y, Peng Y, et al. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis. Cell Death Dis. 2022;13:512 pubmed publisher