This is a knockout-examined antibody summary, based on the publication "Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blots of NLGN2 protein in NLGN2 null mice (KO), NLGN2 heterozygous (HET) and their WT littermates in midbrain. GAPDH is used as loading control. From figure 1 [1]. The band at a higher molecular weight in NLGN2 null lane is thought to be NLGN3.

Antibody: NLGN2

Catalog number: 129211

Summary: Mouse monoclonal IgG1 against neuroligin 1/2/3/4, raised against recombinant mouse neuroligin 3 (cytoplasmic tail, aa 608 - 722). Reacts with rat and mouse. In western blot recognizes neuroligin 2 and 3.

Immunocytochemistry, stains predominantly neuroligin 2 in cultured hippocampal neurons. Detects transiently expressed GFP fusion proteins comprising the cytoplasmic tails of neuroligins 1-4.

Company: Synaptic Systems

Western Blot

Freshly dissected brain tissues, homogenization in RIPA buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% TRITON X-100, 1% sodium deoxycholate, 0.1% SDS) containing protease inhibitors (P8340, 1:100; Sigma, St. Louis, MO, USA).

1:1,000

chemiluminescence (Western Lightning ECL Pro, Perkin Elmer) and X-ray film (Biomax MS, Kodak, 8294985) exposure.