This is a knockout-validated antibody summary, based on the publication "Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blots of whole brain lysate of NLG1 KO and WT mice with indicated antibodies. From [1].

Rabbit polyclonal

Company: Synaptic Systems

Antibody: NLG1

Catalog number: 129 013

Summary: Rabbit polyclonal against Purified recombinant protein of rat neuroligin 1 (cytoplasmic domain; aa 718 - 843). Reacts with rat and mouse by western blot.

Western blot

Whole brain lysate of NLG1 KO and WT mice. Tissues were lysed for 45 min in ice-cold cell lysis buffer containing 20 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3VO4, 20 mM NaF, and 1% protease inhibitor cocktail and phosphatase inhibitor (Roche).

5% dry milk TBST (20 mM Tris base, 9% NaCl, 1% Tween-20, pH 7.6).

1:2,000 dilution in TBST overnight at 4°C.

1:2,000 dilution goat anti-rabbit (A00098, Genscript).

Enhanced chemiluminescence (Thermo Fisher Scientific).