This is a knockout-validated antibody summary, based on the publication "UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Santa Cruz
Antibody: NIRF
Catalog number: sc-398953
Summary: Mouse monoclonal IgG1 against amino acids 144-208 mapping near the N-terminus of human NIRF (UHRF2). Recommended for detection of NIRF of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA
Western blot
Wild type and Uhrf2 knockout MEFs. Cells were lysed with NTN300.
Cell lysates of mouse embryonic fibroblasts (MEF) from wild type (WT) and Uhrf2 knockout (KO) mice were immunoblotted with antibodies as indicated. Intact UHRF2 is absent in Uhrf2 knockout MEF. Please see Figure 1c in the article [1].
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