NIPSNAP1 antibody | knockout validation | Cell Signaling 13226

This is a knockout-validated antibody summary, based on the publication "NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

NIPSNAP1 antibody | knockout validation | Cell Signaling 13226 figure 1
Figure 1. CRISPR-Cas9-mediated double knockout of NIPSNAP1 and NIPSNAP2 (N1/N2 DKO) in HeLa cells were confirmed by immunoblotting. Courtesy from the Authors [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: NIPSNAP1

Catalog number: 13226

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Arg211 of human NIPSNAP1 protein. Reacts with human, mouse, rat and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and NIPSNAP1/2 DKO HeLa cells.

References
  1. Princely Abudu Y, Pankiv S, Mathai B, Håkon Lystad A, Bindesbøll C, Brenne H, et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell. 2019;49:509-525.e12 pubmed publisher